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aureus biofilms were cultured in TSB with or without 2 mg/mL nicotine in glass-bottomed dishes, washed with three times with PBS, and then stained with LIVE/DEAD staining dye [1 M of SYTO9 and 1 M of propidium iodide (PI)] for 20 min

This group also had higher blood cadmium and lead concentrations than nontobacco users