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Passage 1 or 2 cultures were seeded onto collagen IV (C7521, Sigma, St Louis, MO) coated 12 mm Transwell inserts (3460, Corning Costar Corporation, Cambridge, MA) and grown in ALI media to 80% confluence before exposing the apical surface to air

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Vivid Dreams and Nightmares Changes in REM sleep patterns often produce unusually vivid dreams or nightmares during this transition period

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This info is very useful for finding the cause of the symptom triad