Quantitative high throughput screening using a primary human three-dimensional organotypic culture predicts in vivo efficacy
(7)Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- (7) Preparation of Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: (6)Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-1050530105010603DMF3DMF2DMFFmoc-Ala-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- In the Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin in step (6), add the decapping reagent at 10-50C for 5-30 minutes, dry it with a vacuum pump, and re-add the decapping reagent React the reagent at 10-50C for 10-60 minutes, drain it, wash it with isopropanol for 3 times, DMF for 3 times, and distilled DMF for 2 times, add Fmoc-Ala-OH, DIEA, TBTU dissolved in DMF /HOBt or HBTU/HOBt or BOP/HOBt mixture, react at 10-50C for 10-60 minutes, dry with a vacuum pump, wash 3 times with isopropanol, 3 times with DMF, 2 times with re-distilled DMF, Drain, obtain Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin
White, G
Aifm2 blocks ferroptosis independent of ubiquinol metabolism
American Society of Anesthesiologists Chen YH, et al
HughesStovin syndrome (HSS): current status and future perspectives
