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For each biopsy, 57 representative high-power fields (20) were manually selected from tumor and normal epithelial regions using the region of interest (ROI) tool in ImageJ, while avoiding overlapping cells, necrotic zones, and staining artifacts

Secondgeneration vaccines are multicomponent vaccination proteins that allow numerous antigens to approach M (microfold) cells at the same time (Kessans, 2011

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