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Many patients experience what feels like their pre-treatment appetite returning, sometimes described as a switch flipping back on

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Kroenke CH, Caan BJ, Stefanick ML, et al

The eluted proteins were buffer-exchanged to DPBS and concentrated using Amicon Ultra-15 Centrifugation Filter Units (Millipore) with a 30 kDa cutoff by centrifugation at (290400) g at 4 C

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We also confirm that the study was carried out in compliance with the ARRIVE guidelines